Materials and reagents Ammonium bicarbonate, DL-dithiothreitol (DTT), iodoacetamide (IAA) (Sigma-Aldrich, St. Louis, Missouri, USA); mass specification grade Trypsin/Lys-C mixture (Promega, Madison, WI, USA). LC/MS grade water, 0.1% formic acid in acetonitrile (ACN), 0.1% formic acid in water (Thermo Fisher Scientific, Waltham, MA, USA). Acclaim PepMap 100 column, PepMap Trap Cartridge (Thermo Fisher Scientific, Waltham, MA, […]